Figure 6.

Detection of HCV captured by mouse antisera by nested RT-PCR. After immuno capture of HCV from hepatitis C patient serum (1.6 × 10⁷ HCV GEq per mL) by antisera from groups of mice immunized with pcDNA3, pcDNA3SE2-A, pcDNA3SE2-B, pcDNA3SE2-C, pcDNA3SE2-D and pcDNA3SE2-E (lane 1 to lane 6), viruses were detect1ed by nested RT-PCR using the HCV RT-PCR testing kit (Sino-America Co., Shanghai, China). Lane 7, the positive control using McAb against HCV E2 to capture HCV. Lane M, molecular markers.