Figure 3.

Translocation of PKCα protein in the process of apoptosis induced by TPA. A: Redistribution of PKCα protein. Cells were treated with TPA for indicated time, then immunostained with corresponding antibodies or dye as described in Materials and Methods. The images were observed under laser-scanning confocal microscope; B: Effects of various inhibitors, including PKC-specific inhibitor (PIP., 12 μmol/L) and PLC-specific inhibitor (U73122), on redistribution of PKCα. The detecting method was the same as described in Figure 3A; C: Analysis of apoptotic index. The method was similar as in Figure 2B.