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. 2002 Oct 15;8(5):782–786. doi: 10.3748/wjg.v8.i5.782

Table 1.

Nucleotide sequence and size of the expected PCR products for oligonucleotide primers used for RT-PCR

Genes Sequence Size (bp)
c-jun Upstream: 5'-GGAAACGACCTTCTATGACGAGCCC-3' 315
Downstream: 5'-GAACCCCTCCTGCTCATCTGTCAGG-3'
β-actin Upstream: 5'-GTGGGCCGCTCTAGGCACCAA-3' 540
Downstream: 5'-CTCTTTGATGTCACGCACGATTTC-3'

The amplified products were seperated in 20 g•L-1 agorose gel stained with ethidium bromide. After electrophoresis, the gel was observed and photographed under ultraviolet reflector. The density and area of each band were analyzed using ChemiImagerTM 4000 Digital System (Alpha Innotech Corporation, USA).