Table 3.
Expression of p16INK4A gene in SW1116 and Colo-320 cells (the band density)
| 5-aza-dC treatment | Mock treated | 2 μmol/L, 24 h | 5 μmol/L, 24 h | 10 μmol/L, 24 h | 2 μmol/L, 72 h | 5 μmol/L, 72 h | 10 μmol/L, 24 h |
| SW1116 | 1494.7 | 2055.5 | 2436.9 | 3487.3 | 1592.0 | 2074.8 | 2774.0 |
| Colo-320 | 809.1 | 860.6 | 829.2 | 1298.8 | 875.7 | 923.5 | 1189.6 |
The density of each band from RT-PCR in each lane of Figure 3 was normalized to the amount of total RNA as determined by the density of band in RT-PCR for β-actin.