Skip to main content
. 2015 Sep 15;5(10):3149–3161.

Figure 4.

Figure 4

Effects of F56 on VEGFR1, VEGFR2, PI3K and AKT signaling pathways. A. HUVECs seeded on coverslips were transfected with indicated siRNAs for 48 hr, treated with FITC-F56 (green) for 30 min, followed by immunostaining of VEGFR1 (red) and counterstaining of DNA with DAPI (blue). B. HUVECs were transfected with indicated siRNAs for 48 hr, followed by Western blotting. C. HUVECs were starved overnight, after the treatment of F56 or scr-F56 at the concentration of 50 g/mL for 30 min, 10 ng/mL VEGF-A was added to the medium for 10 min stimulation, and the expression of indicated proteins were assayed by western blotting.