Figure 5.

AAL activates Mincle/Syk/Card9 signaling to couple with Nlrp3 inflammasome assembly. The above prepared BMDM lysates were next subjected to Western blot analysis of Mincle down-stream molecules Syk and Card9 as well as Nlrp3 inflammasome assembly. A. Western blot analysis for the levels of Syk/Card9 signaling in AAL-stimulated BMDMs. Left: a representative Western blot result; right: results with 4 replications. B. Mincle/Syk/Card9 signaling couples to the Nlrp3 inflammasome assembly. Left: a representative result for Western blot analysis of Nlrp3, pro-IL-1β, pro-Casp1 and cleaved Casp1 (p20); right: Bar graphics figures showing the results with 4 replications. Four samples were analyzed in each study group, and the data were expressed as mean ± SEM and analyzed by two-way ANOVA, ***P < 0.001 compared with the value in control group.