Figure 6.

AAL modulates chromatin structure to regulate Hmgb1 secretion. A. AAL significantly stimulated H3K4 di-/trimethylation in macrophages. Left: a representative result for Western blotting; right: bar graphic figures showing the data with 4 replications. B. Cytoplasmic Hmgb1 levels in macrophages following AAL stimulation. Left: a representative Western blot result; right: results with 4 replications. C. Nuclear Hmgb1 levels in macrophages following AAL stimulation. Left: a representative result for Western blotting; right: data obtained with 4 replications. D. Hmgb1 immunostaining in macrophages following AAL stimulation. AAL stimulated a significant Hmgb1 cytoplasmic translocation. E. Western blot analysis of Hmgb1 in AAL-stimulated macrophage culture supernatant. Left: a representative Western blot result; right: results with 4 replications. Four samples were analyzed in each study group, and the data were analyzed by two-way ANOVA followed by Bonferroni test and expressed as mean ± SEM. **P < 0.01, ***P < 0.001 compared with the value in control group.