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. 2015 Oct 15;7(10):1952–1962.

Figure 5.

Figure 5

GADD45β knockdown fails to restore the effects of miR-1 inhibition on the cardiac infarct area and cardiomyocytes apoptosis after I/R or H/R injury. A. Western blot assay of GADD45β protein expression in rats treated with GADD45β siRNA, or co-treated with miR-1 ASO and siRNA against GADD45β. GAPDH wasa loading control. B. Statistical analysis of IA/AAR ratio in rats treated with GADD45β siRNA, or co-treated with miR-1 ASO and siRNA against GADD45β. IA, infarct area; AAR, area at risk. C. Effects of miR-1 inhibition on the apoptotic cells in cardiac tissues subjected to I/R injury were investigated by TUNEL staining. Graph (below) represents the percentage of apoptotic cells. D. Western blot assay of GADD45β protein expression in cardiomyocytes transfected with GADD45β siRNA, or co-treated with miR-1 ASO and siRNA against GADD45β. GAPDH wasa loading control. E. Effects of miR-1 inhibition on the apoptosis of cardiomyocytes subjected to H/R injury were investigated by TUNEL staining. Apoptotic cells were stainedred. *P<0.05.