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. 2015 Oct 15;7(10):1963–1973.

Figure 1.

Figure 1

Ectopic expression of NRASG12D in CD34+ human cord blood SPCs. A. Schematic diagram of experimental strategy. We overexpressed NRASG12D in the cord blood CD34+ progenitors using lentiviral transduction, and evaluated its effects in vitro (CFU assay) and in vivo (transplantation). B. Schematic picture of lentiviral constructs. The promoter region is shown in black. C. Sequencing of NRASG12D mutation using forward primer. Arrow indicates the mutation of nucleotide G to A at the codon 12. D. Transduction efficiency of cord blood CD34+ cells after 48 hours incubation with viruses. E. Confirmation of ectopic expression f NrasG12D at the mRNA level. Total RNA was extracted from CD34+ hSPCs transduced with NRASG12D or GFP control viruses. Data are shown as mean + SD (**, P<0.001). Triplicates from one representative experiment are shown.