Figure 2.

Overexpression of NRASG12D in CD34⁺ hSPCs enhances CFU-M formation and inhibits BFU-E formation. CD34⁺ hSPCs were infected with NRASG12D and GFP viruses. Two days after viral transduction, 1000 GFP⁺ cells were sorted and plated in semisolid medium. (A) Colonies were numerated after 13 days in culture (n=3). *P<0.05, **P<0.01 and ***P<0.001. (B, C) Representative images of different CFU subtypes from cells transduced with NRASG12D (B) or GFP control viruses (C).