Figure 3.

NRASG12D-expressing CD34⁺ hSPCs show bias of myelopoiesis in vivo. Sublethally irradiated (1.5 Gy) immunodeficient mice were transplanted with 3×10⁵ total CD34⁺ cord blood cells infected with NRASG12D or GFP control viruses. The transduction efficiencies were adjusted to ~50%. Samples were collected from NRASG12D moribund recipients. Debris and dead cells (DAPI positive) were excluded from analysis. A. Engraftment analysis of hematopoietic lineages in peripheral blood of recipients six weeks after transplantation. B. Flow cytometric analysis of human white blood cells in bone marrow and spleens from a representative moribund mouse. Markers used: B-lymphoid cells, CD19+; myeloid cells, CD33⁺; and T-lymphoid cells, CD3/4/8⁺. C. Statistical analysis of GFP⁺ and GFP^- human myeloid (CD33⁺) cells in peripheral blood of transplanted recipeints. D. Statistical analysis of GFP⁺ and GFP^- human myeloid (CD33⁺) cells in BM and spleens of transplanted NSI mice (n=3, *, P<0.05).