Figure 5.

Hyper-activation of AKT, MAPK and STAT5 pathways in NRASG12D-overexpressing CD34⁺ hSPCs. CD34⁺ enriched cord blood cells were transduced with GFP and NRASG12D viruses. Two days after viral transduction, cells were immediately fixed, permeablized and analyzed using flow cytometry. A. NRASG12D expressing hSPCs were gated as GFP⁺ CD34⁺ CD38^- cells. B. Representative plots of phosphorylated AKT, ERK1/2 and STAT5. Levels of phosphorylated AKT, ERK1/2 and STAT5 were shown from cells expressing GFP control and NRASG12D. C. Statistical analysis of the levels of phosphorylated AKT, ERK1/2 and STAT5. Median intensities of p-AKT, p-ERK1/2 and p-STAT5 were analyzed from individual samples. For each experiment, CD34⁺ hSPCs samples were isolated from the same donor to reduce variability. Data from one representative experiment with three replicates were shown as mean + SD. *P<0.05, and **P<0.01.