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. 2015 Oct 15;7(10):2053–2059.

Figure 3.

Figure 3

miR-205 negatively regulated CREB1 by binding to the CREB1 3’UTR. A. The potential miR-205 binding sequence of CREB1 3’UTR and the mutant. B. A luciferase reporter assay showed the inhibitory effect of miR-205 on CREB1 3’UTR luciferase activity in HEK293 cells. C. CREB1 protein levels were analyzed by Western blot in SW480 and HT29 cells transfected with miR-205 mimics or negative control (NC). D. Relative expression levels of CREB1 in CRC tissues and adjacent non-tumor tissues. E. Correlation of CREB1 expression to miR-205 expression in 20 CRC tissues using simple linear regression analysis. P < 0.05.