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. 2015 Oct 15;7(10):2115–2126.

Figure 3.

Figure 3

SOCS6 is a target of miR-155 in breast cancer cells. A. The miR-155 and the miR-155-binding site in the 3’UTR of SOCS6. B. Luciferase reporter assay with cotransfection of wild-type or mutant 3’UTR (100 ng) and miR-155 or miR-control in MCF7 cells. Firefly luciferase activity of each sample was normalized against Renilla luciferase activity. C. The effects of miR-155 on the expression of endogenous SOCS6 and SOCS6 mRNA in MCF7 and SKBR3 cells was tested by real time RT-PCR. D. The effects of anti-miR-155 on the expression of endogenous SOCS6 in MCF7 and SKBR3 cells. miR-155 was tested by real time RT-PCR. E. Western blot was used for monitoring the SOCS6 expression in MCF-7 and SKBR3 cells after the transfection for 48 h with miR-155 or anti-miR-155. All data from three separate experiments are presented as mean ± SD. **P<0.01 vs the control.