Figure 4.

SOCS6 makes breast cancer cells become sensitive to tamoxifen. A. MCF-7, MCF-7/TAM cells, SKBR3 and SKBR3/TAM cells were infected with LV-SOCS6 or its control and total protein was extracted for immunoblotting. B and C. MCF-7, MCF-7/TAM cells, SKBR3 and SKBR3/TAM cells were infected with LV-SOCS6 using colony formation assay to test. D. MCF-7/TAM cells were infected with LV-SOCS6 and treated with TAM, and apoptosis was quantitated using flow cytometry (left panel). The right panel was quanlification of apoptosis rate from flow cytometry. E. MCF-7, MCF7/TAM, SKBR3 and SKBR3/TAM cells were infected with LV-SOCS6 or its control and total RNA was extracted for miR-155 expression by real time RT-PCR. Data is represented as mean ± SE of three independent experiments. **P<0.01.