Skip to main content
. 2015 Nov 17;17(10):776–788. doi: 10.1016/j.neo.2015.10.002

Figure 4.

Figure 4

Ccl5 expression is increased in murine OPGs and human PAs. (a) Immunofluorescence of murine optic nerve tissue reveals increased Ccl5 (green) protein expression in Nf1 optic glioma specimens (FMC, n = 5) relative to control optic nerve tissue (FM, n = 5). Quantification is represented in the adjacent graph. Each error bar represents the mean ± SEM. Asterisks denote statistically significant differences: (**) P = .0079. (b) Double labeling of Ccl5 and Iba1 reveals that the chemokine Ccl5 is expressed in Iba1+ cells (microglia). Scale bar, 50 μm. DAPI (blue) was used as a counterstain to identify all cells in the sections. Representative images are shown, with insets demonstrating immunopositive cells (arrows). (c) In the GSE44971 GEO dataset, CCL5 RNA expression in human PAs (n = 46 sporadic tumors and 3 from individuals with NF1) was increased for all three CCL5 probe sets relative to normal brain (n = 9). The white bars denote normal brain samples, the black bars denote the sporadic PA specimens, and the hatched bars denote the NF1-associated PAs. Each error bar represents the mean ± SD. (**) P = .0015 using a one-way analysis of variance with Bonferroni posttest correction. (d) In the GSE42656 GEO dataset, CCL5 RNA expression was also increased for both CCL5 probe sets. The white bars denote normal brain samples (n = 16), whereas the black bars denote the PA specimens (n = 14). Each error bar represents the mean ± SD. (***) P < .0001 using a two-way analysis of variance with Bonferroni post-test correction.