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Ccl5 increases optic nerve astrocyte proliferation in vitro. Following incubation with 250 ng of murine recombinant Ccl5, there was an increase in Nf1-deficient astrocyte proliferation (Ki67 labeling) relative to vehicle treatment in vitro. Representative Ki67+ proliferating cells (green) and DAPI+ nuclei (blue) are shown. Arrowheads indicate Ki67-positive cells. Scale bar, 100 μm. Each error bar represents mean ± SEM. Asterisks denote statistically significant differences.
