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. 2015 Nov 17;17(10):776–788. doi: 10.1016/j.neo.2015.10.002

Figure 6.

Figure 6

Ccl5 is necessary for murine Nf1 optic glioma growth in vivo. (a) At 3 months of age, FMC mice received i.p. injections of either Ccl5 neutralizing antibodies (n = 5 mice) or an IgG2A isotype-matched control antibody (n = 5 mice) every day for 2 weeks. Three hours before euthanasia, mice were i.p. injected with 50 mg/kg of BrdU. Ccl5 treatment decreased (b) murine Nf1 optic glioma proliferation as measured by BrdU incorporation and (c) the percentage of Iba1+ microglia. Scale bar, 100 μm. (d) Following anti-Ccl5 treatment, TUNEL staining (n = 5 mice/group; top panels) and Brn3a immunofluorescence (n = 5 mice/group; bottom panels) revealed decreased retinal cell apoptosis (%TUNEL+ cells) and increased RGC numbers (Brn3a+ cells) relative to IgG-treated controls. Representative individual TUNEL+ and Brn3a+ cells within the ganglion cell layer are shown in the insets. Quantification is represented in the adjacent graphs. Scale bar, 50 μm. Each error bar represents the mean ± SEM. Asterisks denote statistically significant differences. INL, inner nuclear layer; GCL, ganglion cell layer; ONL, outer nuclear layer.