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. 2015 Nov 24;6:1310. doi: 10.3389/fmicb.2015.01310

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Copyright © 2015 Kuepper, Dickler, Biggel, Behnken, Jäger, Wierckx and Blank.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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oAB production profiles of various P. putida KT2440 strains in shake flasks. Biomass growth (A) and oAB production (B) of the following Pseudomonas putida KT2440 strains in an initial screening experiment: , ΔtrpDC pSEVA234_trpE^S40FG; , ΔtrpDC pSEVA234_aroG^D146N; , ΔtrpDC pSEVA234_aroG^D146N_trpE^S40FG; , ΔtrpDC ΔpheA pSEVA234_trpE^S40FG; , ΔtrpDC ΔpheA pSEVA234_aroG^D146N; , ΔtrpDC ΔpheA pSEVA234_aroG^D146N_trpE^S40FG; , pSEVA234_aroG^D146N_trpE^S40FG. All cultures were performed in mineral medium with 20 mM initial glucose concentration and addition of tryptophan and/or phenylpyruvate as described above. The arrows indicate the addition of glucose to a concentration of 20 mM.

Inline graphic — oAB production profiles of various P. putida KT2440 strains in shake flasks. Biomass growth (A) and oAB production (B) of the following Pseudomonas putida KT2440 strains in an initial screening experiment: , ΔtrpDC pSEVA234_trpE^S40FG; , ΔtrpDC pSEVA234_aroG^D146N; , ΔtrpDC pSEVA234_aroG^D146N_trpE^S40FG; , ΔtrpDC ΔpheA pSEVA234_trpE^S40FG; , ΔtrpDC ΔpheA pSEVA234_aroG^D146N; , ΔtrpDC ΔpheA pSEVA234_aroG^D146N_trpE^S40FG; , pSEVA234_aroG^D146N_trpE^S40FG. All cultures were performed in mineral medium with 20 mM initial glucose concentration and addition of tryptophan and/or phenylpyruvate as described above. The arrows indicate the addition of glucose to a concentration of 20 mM.