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. 2015 Nov 24;6:1310. doi: 10.3389/fmicb.2015.01310

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Copyright © 2015 Kuepper, Dickler, Biggel, Behnken, Jäger, Wierckx and Blank.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tryptophan-limited fed-batch cultures of P. putida KT2440 ΔtrpDC pSEVA234_aroG^D146N_trpE^S40FG in controlled bioreactors with different feeding approaches. (A) Feed molar ratio of glucose to tryptophan 1,000:1. (B) Feed molar ratio of glucose-to-tryptophan 2,000:1. , cell dry weight; , oAB concentration. The arrows indicate the start of the feed after the initial batch phase.

Inline graphic — Tryptophan-limited fed-batch cultures of P. putida KT2440 ΔtrpDC pSEVA234_aroG^D146N_trpE^S40FG in controlled bioreactors with different feeding approaches. (A) Feed molar ratio of glucose to tryptophan 1,000:1. (B) Feed molar ratio of glucose-to-tryptophan 2,000:1. , cell dry weight; , oAB concentration. The arrows indicate the start of the feed after the initial batch phase.