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. 2015 Nov 24;6:1299. doi: 10.3389/fmicb.2015.01299

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Copyright © 2015 Dong, Zhu, Chen, Ye and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overproduction of endolysin P28 in Escherichia coli BL21 (DE3) cells. Shown is SDS-PAGE (15%) of the cellular proteins from 80 μL cultures of bacteria. The gel was stained by Coomassie brilliant blue R-250 to visualize the bands. E. coli BL21 (DE3; pET-26b-ORF8) before induction (lane 1) and 6 h after induction (1 mM IPTG; lane 2). The overexpressed oligohistidine-tagged endolysin P28 was purified (lane 3). M, molecular masses of reference proteins (Thermo Scientific; in kilodaltons).