FIGURE 3.

Lytic activity of different concentration endolysin P28 against Stenotrophomonas maltophilia P28. One hundred microliter purified endolysin P28 was mixed with 300 μL cell suspension (OD₆₀₀ 0.5 ± 0.05). The concentration of the purified endolysin was changed while the reaction volumes of purified endolysin (100 μL) and cell suspension (300 μL) remained unchanged. After incubation for 1 h at 30°C, the CFUs of the mixture was determined and the antibacterial activity was calculated as described in the section “Materials and Methods”. The data were statistically analyzed by using the Origin8.1 program. All experiments were repeated at least three times. Mean values (±SD) are shown.