Figure 1.

Leakage of vesicles composed of 30 μM POPC-containing cholesterol (CHOL) induced by surfactins (SF)/iturins (IT)/fengycins (FE) natural mixture (left: A/D), FE (center: B/E), and SF (right: C/F), respectively. (A–C) Dye efflux after 1 h, E, as a function of lipopeptide concentration. Dotted lines are to guide the eye only. (D–F) Lifetime of entrapped dye, τ_E, as a function of lipopeptide concentration. Solid lines simulate τ_E for ideal homogeneously graded leakage (Eqs. 3 and 4). For clarity, only τ_E values of 10 or 20 mol% CHOL are shown. Refer to Fig. S5 for τ_E at other sterol contents. Squares, diamonds, triangles, and circles represent pure POPC, 10, 20, and 30 mol% CHOL, respectively. To see this figure in color, go online.