Figure 4.

Model-fitting estimates of a fluorescent protein layer radius in B. megaterium and B. subtilis, and in simulated calibration data with a ground-truth radius of 500 nm (Cal). (a) The average layer radius was obtained by analyzing at least three separate fields of typically 200 spores each, and the error bars indicate the standard deviation of these measurements. For the ellipsoidal models, the radius of a sphere of equal volume is presented. (b) Residual errors of fitted models. (c) The distribution of radii fitted to SleL and CotG in 200 individual B. subtilis spores. (d) The aspect ratio of ellipsoidal B. subtilis spores. (e) The polarity parameter quantifying the tendency of proteins to localize preferentially at the spore poles. To see this figure in color, go online.