Figure 5.

Superresolved reconstruction of fluorescence intensity in B. subtilis spores. (a) The parameters fitted to SleL-GFP B. subtilis images are fed back into the ellipsoid model for its image, while decreasing the inferred PSF to remove instrumental blurring. The original widefield image data are shown to the left of the reconstruction for comparison. (b and c) The inferred parameters of multiple protein layers are averaged and visualized in the same way to illustrate the structural details captured by model fitting for B. megaterium (b) and also for B. subtilis (c). Scale bars, 2 μm (a) and 1 μm (b and c).