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Effect of food pH on Drosophila adult survival. (A) Buffering capacity—volume of acid required to change pH of indicated dietary component by 1 unit. (B) Lifespan on YE diet buffered to indicated pH (Expt. 1; n = 114–122 flies per group). (C–G) Lifespan on phosphate-buffered YE (C), sucrose-only (D–F), or agar-only (G) diet [Expt. 2; n = 115–136 (C–F) or 61–63 flies per group (G)]. Labeled curves without a common letter differ, P < 0.05. CM, cornmeal; S, sucrose; YE, yeast extract.
