Fig. 3.

AFN exposure increases antioxidant gene expression in zebrafish embryos. The levels of (A) eif1a (reference), (B) gstp1, (C) prdx1, and (D) gclc mRNA was determined from pools of 50 embryos in each treatment group at 1 and 2 dpf. AFN exposure began at 6 hpf and was either continued or washed out at 1 dpf. The C_t was normalized to the reference genes and scaled to the solvent control at each time point as a fold change. The values represent the mean ± SEM from three biological replicates. Statistically significant differences are noted as ^*p≤0.05, ^**p≤0.01, ^***p≤0.001, and ^****p≤0.0001.