Fig. 1.
Electrophysiological characteristics of Cav channels in IonWorks™ Barracuda. (A, B) Voltage-dependent activation of channels. Calcium current families, where 7 mM Ca2+ is the charge carrier: Cav1.2 (i), Cav2.1 (ii), Cav2.2 (iii), and Cav3.2 (iv). Currents were elicited by applying test pulses from −80 to +60 mV in 10 mV increments; the holding potential of −80 mV for Cav1.2 and −90 mV for other channels. In case of Cav3.2, a 500-ms conditioning voltage prepulse to −120 mV preceded each test pulse. The averaged current–voltage relationship is shown in (B) (mean±SE; n=16). Superimposition of steady-state inactivation curves of Cav channels is illustrated in (C). The currents were elicited by 400 ms test pulses, after 1-s, the conditioning prepulses (CPs) ranged from −120 to 50 mV (10 mV increments); the test pulse potentials were 10, 10, 30, and −30 mV for Cav1.2, Cav2.1, Cav2.2, and Cav3.2 channels, respectively.