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Multiple mode voltage protocol for evaluation of voltage- and use-dependent block in Cav channels. (A) Optimized voltage protocol to detect use and voltage dependence of test compound interactions in Cav channels. (B) Representative Cav2.2 current traces elicited with the voltage protocols before (baseline) and after addition of vehicle, 10 μM mibefradil, and 30 μM verapamil.
