Table 5.
Assay Quality Parameters
| Ion channel | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cav1.2 | Cav2.1 | Cav2.2 | Cav3.2 | |||||||||
| Parameter | Pre-pulse−50 mV | Pre-pulse −90 mV | 0.1 Hz (−90 mV) | Pre-pulse−50 mV | Pre-pulse −90 mV | 0.1 Hz (−90 mV) | Pre-pulse −50 mV | Pre-pulse −90 mV | 0.1 Hz (−90 mV) | Pre-pulse−75 mV | Pre-pulse −90 mV | 1 Hz (−90 mV) |
| RD, %a | 43.9±1.4 | N/A | N/A | 26.1±7.6 | N/A | N/A | 36.4±4.5 | N/A | N/A | 28.8±12.6 | N/A | N/A |
| Z′ | 0.55±0.09 | 0.56±0.05 | 0.40±0.06 | 0.57±0.04 | 0.61±0.06 | 0.46±0.05 | 0.69±0.03 | 0.64±0.09 | 0.42±0.09 | 0.65±0.11 | 0.72±0.03 | 0.64±0.06 |
| SW | 4.0±1.4 | 4.5±0.6 | 3.3±1.9 | 5.2±1.2 | 5.9±1.4 | 3.1±0.8 | 6.4±3.5 | 4.3±1.0 | 3.0±0.9 | 6.1±2.2 | 10.9±2.5 | 9.1±2.9 |
Reference compounds for calculating Z′ and SW were verapamil (Cav1.2, Cav2.1, Cav2.2) or mibefradil (Cav3.2).
a
RD was measured at TP1 in time-matched vehicle control wells.
RD, run down; TP1, test pulse 1.