Table 1.
Identified proteins showing significant differential abundance between young and senescent HPMC cells (p < 0.05) with references of their predicted or reported O-GlcNAcylated sites.
| Protein name | Gene name | SwissProt entry name |
MW (kD)a |
pIb | Lengthc | Spotd | Potential O-GlcNAc sitese | Se | Te | Predicted O-GlcNAc sitesf | Sf | Tf | Referencesg |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin, cytoplasmic 2 | ACTG1 | ACTG_HUMAN | 41.8 | 5.31 | 375 | 294 | 51 | 25 | 26 | 9 | 8 | 1 | [43] |
| Actin, cytoplasmic 1 | ACTB | ACTB_HUMAN | 41.7 | 5.29 | 375 | 294 | 51 | 25 | 26 | 9 | 8 | 1 | [38, 40, 44] |
| Keratin, type II cytoskeletal 7 | KRT7 | K2C7_HUMAN | 51.4 | 5.39 | 469 | 52 | 63 | 46 | 17 | 4 | 4 | 0 | —∗ [36–38, 44] |
| Cofilin-2 | CFL2 | COF2_HUMAN | 18.7 | 7.88 | 166 | 198 | 20 | 12 | 8 | 4 | 4 | 0 | [24] |
| Transgelin-2 | TAGLN2 | TAGL2_HUMAN | 22.4 | 8.45 | 199 | 173 | 17 | 7 | 10 | 2 | 2 | 0 | [41, 43] |
| 60 kDa heat shock protein, mitochondrial | HSPD1 | CH60_HUMAN | 61.0 | 5.24 | 573 | 19 | 14 | 6 | 8 | 6 | 4 | 2 | [37, 39, 41, 42, 44] |
| Heat shock cognate 71 kDa protein | HSPA8 | HSP7C_HUMAN | 70.9 | 5.37 | 646 | 16 | 82 | 35 | 47 | 4 | 2 | 2 | [36–38, 41, 43–45] |
| Proteasome subunit beta type-2 | PSMB2 | PSB2_HUMAN | 22.8 | 6.52 | 201 | 180 | 18 | 10 | 8 | 9 | 5 | 4 | [41, 43] |
| Proteasome subunit beta type-3 | PSMB3 | PSB3_HUMAN | 22.9 | 6.12 | 205 | 150 | 20 | 7 | 13 | 2 | 0 | 2 | —∗ [41, 43] |
| Nucleoside diphosphate kinase A | NME1 | NDKA_HUMAN | 17.1 | 5.82 | 152 | 198 | 11 | 6 | 5 | 3 | 2 | 1 | [41] |
| 5′(3′)-deoxyribonucleotidase, cytosolic type | NT5C | NT5C_HUMAN | 23.4 | 6.18 | 201 | 150 | 14 | 6 | 8 | 1 | 0 | 1 | — |
aRelative molecular mass of the protein as calculated from the amino acid sequence of the polypeptide without any co- or posttranslational modifications; bcalculated pI of the protein as obtained from SwissProt database; cnumber of amino acids in the protein sequence; dspots numbered according to Figure 2; epotential O-GlcNAcylation sites expressed as the total number of serine (S) and threonine (T) residues in the protein sequence; fpredicted O-GlcNAcylation sites expressed as the number of predicted serine (S) and threonine (T) residues in the protein, obtained from dbOGAP database using default settings in OGlcNAcScan tool; greferences within protein candidates that have been reported to be O-GlcNAcylated. ∗proteins from the same protein family of the candidate have been reported to be O-GlcNAcylated. [dbOGAP: http://cbsb.lombardi.georgetown.edu/hulab/OGAP.html].