Figure 2.

Effect of shikonin on the expression of lncRNA-NR024118, SOCS3, inflammatory cytokines, and MMPs in synovial fibroblasts. On study day 10, hind limbs from mice treated with shikonin were harvested and homogenized. Synovial fibroblasts were isolated from these samples, total RNA was extracted, and then the expression of lncRNAs in synovial fibroblasts from mice treated with shikonin was determined as demonstrated by the microarray analysis (a). The mRNA levels of SOCS3 (b), IL-6, IL-8, MMP-1, and MMP-3 (c) were determined with RT-RCR. The data are presented as the mean ± SD of five determinations. ^∗ P < 0.05, ^∗∗ P < 0.01 versus control.