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. 2015 Nov 10;2015:631737. doi: 10.1155/2015/631737

Figure 4.

Figure 4

Effect of TSA on the expression of NR024118 and the application of Shikonin on histone acetylation at NR024118 promotor. Synovial fibroblasts from RA patients were treated with TSA (0, 0.1, 0.5, and 1.0 μM) for 24 h. Gene expression of NR024118 was determined by RT-RCR and all RNA values were normalized to β-actin mRNA expression. This experiment was repeated three times (a). Histone acetylation was determined by real-time PCR analysis of chromatin immunoprecipitates from RA synovial fibroblasts. Real-time PCR values were determined by reference to a standard curve generated by real-time PCR amplification of genomic DNA using NR024118 promoter primers. Each value was normalized to the total amount of NR024118 promoter DNA added to the immunoprecipitation reaction. Fold increases compared to untreated cells are shown; representative data from at least three separate chromatin preparations are shown (b). ∗ P < 0.05, ∗∗ P < 0.01 versus control.