Table 1. The kinetic parameters for nsP2 pro Wild Type and mutants.
| nsP2 pro | kcat (×10−4 s−1) | Km (μM) | kcat/Km (×10−4 s−1∙μM−1) |
|---|---|---|---|
| Substrate AGA | |||
| Wild Type | 46.16 ± 8.91 | 65.73 ± 17.08 | 0.722 ± 0.151 |
| Cys478Ala | 5.150 ± 1.533 | 3.293 ± 1.048 | 1.572 ± 0.197 |
| Ser482Ala | 16.35 ± 2.91 | 10.40 ± 2.02 | 1.637 ± 0.541 |
| Trp549Ala | 32.47 ± 7.61 | 30.14 ± 9.84 | 1.04 ± 0.35 |
| Trp549Phe | 7.59 ± 4.40 | 4.14 ± 2.02 | 1.77 ± 0.18 |
| Substrate AGC | |||
| Wild Type | 24.21 ± 4.86 | 6.957 ± 1.607 | 3.614 ± 1.106 |
| Cys478Ala | 31.49 ± 12.71 | 18.43 ± 16.73 | 2.417 ± 1.220 |
| Ser482Ala | 21.25 ± 11.55 | 10.92 ± 2.80 | 1.871 ± 0.577 |
| Trp549Ala | 74.89 ± 12.84 | 7.66 ± 4.56 | 11.57 ± 5.20 |
| Trp549Phe | 12.66 ± 0.53 | 4.09 ± 1.56 | 3.36 ± 1.09 |
| Substrate AGG | |||
| Wild Type | 16.06 ± 11.45 | 8.618 ± 5.027 | 1.792 ± 0.431 |
| Cys478Ala | 15.07 ± 7.70 | 7.172 ± 3.435 | 2.243 ± 0.854 |
| Ser482Ala | 14.33 ± 6.52 | 5.807 ± 3.646 | 3.080 ± 1.598 |
| Trp549Ala | 16.65 ± 12.39 | 26.87 ± 9.67 | 0.67 ± 0.48 |
| Trp549Phe | 13.43 ± 1.66 | 7.59 ± 1.28 | 1.79 ± 0.24 |
The kinetic results are represented as mean ± S.D. for at least 3 independent experiments.