Table 3. The remaining percent activity of enzyme in the presence of metal ions.
| nsP2 pro | AGA | AGC | AGG |
|---|---|---|---|
| Nickel | |||
| Wild Type | 46.50 ± 4.91 | 50.14 ± 7.61 | 53.26 ± 7.80 |
| Cys478Ala | 50.77 ± 29.06 | 65.99 ± 42.26 | 76.73 ± 38.25 |
| Ser482Ala | 59.79 ± 8.07 | 95.79 ± 7.42 | 92.02 ± 15.23 |
| Trp549Ala | 75.05 ± 8.95 | 60.20 ± 9.80 | 37.35 ± 8.09 |
| Trp549Phe | 89.49 ± 13.00 | 67.96 ± 14.34 | 67.00 ± 5.83 |
| Copper | |||
| Wild Type | 82.65 ± 4.02 | 19.70 ± 8.70 | 77.41 ± 8.21 |
| Cys478Ala | 103.6 ± 65.5 | 31.49 ± 20.18 | 87.71 ± 39.68 |
| Ser482Ala | 86.42 ± 42.16 | 34.39 ± 8.24 | 112.6 ± 40.6 |
| Trp549Ala | 83.09 ± 8.99 | 8.27 ± 2.39 | 68.68 ± 4.85 |
| Trp549Phe | 81.46 ± 12.00 | 25.93 ± 1.97 | 60.33 ± 7.26 |
| Cobalt | |||
| Wild Type | 200.0 ± 11.2 | 59.63 ± 0.75 | 119.3 ± 9.7 |
| Cys478Ala | 313.8 ± 52.5 | 55.63 ± 8.71 | 114.0 ± 47.2 |
| Ser482Ala | 103.1 ± 2.8 | 52.70 ± 3.06 | 123.1 ± 22.4 |
| Trp549Ala | 161.98 ± 7.12 | 34.44 ± 11.43 | 140.67 ± 20.07 |
| Trp549Phe | 283.39 ± 44.92 | 58.52 ± 6.21 | 150.07 ± 15.84 |
| Magnesium | |||
| Wild Type | 83.03 ± 9.53 | 123.7 ± 44.4 | 111.2 ± 6.6 |
| Cys478Ala | 152.4 ± 49.1 | 120.9 ± 18.0 | 121.2 ± 36.1 |
| Ser482Ala | 105.6 ± 6.0 | 111.3 ± 8.7 | 107.9 ± 7.4 |
| Trp549Ala | 97.10 ± 1.61 | 91.69 ± 5.82 | 92.07 ± 4.99 |
| Trp549Phe | 108.84 ± 5.33 | 104.89 ± 2.92 | 93.33 ± 4.81 |
| Zinc | |||
| Wild Type | 39.23 ± 26.94 | 38.76 ± 6.50 | 39.63 ± 11.55 |
| Cys478Ala | 60.38 ± 44.37 | 44.25 ± 5.36 | 58.13 ± 50.61 |
| Ser482Ala | nd | 21.61 ± 12.89 | 59.06 ± 11.04 |
| Trp549Ala | 71.37 ± 5.84 | 55.59 ± 4.39 | 74.17 ± 12.27 |
| Trp549Phe | 93.37 ± 2.65 | 48.88 ± 10.47 | 71.32 ± 13.34 |
Percent remaining activity of protease compared to protease control with no metal present. Values are means ± S.D. with n ≥ 3. Nd indicates activity could not be detected, that is, complete inhibition.