. 2015 Nov 26;6(4):366–378. doi: 10.4331/wjbc.v6.i4.366

Table 1.

Mutations introduced to glyceraldehyde 3-phosphate dehydrogenase cDNA ORF

GAPDH variant	Mutagenic primers¹	Nucleotide change	Codon change
Y94A	CGATGCTGGCGCTGAGGCCGTCGTGGAGTCCACTGG	280-281	UAC- > GCC
Y94A	CCAGTGGACTCCACGACGGCCTCAGCGCCAGCATCG	280-281	UAC- > GCC
S98A	GAGTACGTCGTGGAGGCCACTGGCGTCTTCACC	290	UCC- > GCC
S98A	GGTGAAGACGCCAGTGGCCTCCACGACGTACTC	290	UCC- > GCC
T99A	AGTACGTCGTGGAGTCCGCTGGCGTCTTCACCACC	295	ACU- > GCU
T99A	GGTGGTGAAGACGCCAGCGGACTCCACGACGTACT	295	ACU- > GCU
T99I	GAGTACGTCGTGGAGTCCATTGGCGTCTTCA	296	ACU- > AUU
T99I	TGAAGACGCCAATGGACTCCACGACGTACTC	296	ACU- > AUU

Mutated nucleotides are indicated in bold font; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.