Table 3.
Fluorescence recovery after photobleaching analysis of dynamic properties of wild type and mutated enhanced green fluorescent protein-glyceraldehyde 3-phosphate dehydrogenase
| Protein | Compartment | (1-Mf) | t1/2, s | D, μm2/s |
| EGFP-GAPDH-WT | cyto | 0.18 ± 0.038 | 0.19 ± 0.055 | 27.17 ± 1.632 |
| EGFP-GAPDH-Y94A | cyto | 0.17 ± 0.129 | 0.09 ± 0.014 | 43.76 ± 7.158 |
| EGFP-GAPDH-S98A | cyto | 0.20 ± 0.009 | 0.12 ± 0.016 | 29.15 ± 3.885 |
| EGFP-GAPDH-T99A | cyto | 0.11 ± 0.062 | 0.11 ± 0.023 | 35.52 ± 8.133 |
| EGFP-GAPDH-T99I | cyto | 0.17 ± 0.06 | 0.22 ± 0.041 | 17.46 ± 1.733 |
| EGFP-GAPDH-WT | nuclei | 0.62 ± 0.048 | 1.41 ± 0.033 | 2.91 ± 0.331 |
| EGFP-GAPDH-Y94A | nuclei | 0.23 ± 0.127 | 0.09 ± 0.034 | 39.05 ± 6.291 |
| EGFP-GAPDH-S98A | nuclei | 0.35 ± 0.136 | 0.13 ± 0.018 | 26.57 ± 3.747 |
| EGFP-GAPDH-T99A | nuclei | 0.38 ± 0.120 | 0.11 ± 0.027 | 33.01 ± 9.209 |
| EGFP-GAPDH-T99I | nuclei | 0.29 ± 0.028 | 0.21 ± 0.059 | 14.97 ± 1.855 |
Cyto: Cytoplasmic compartment; nuclei: Nuclear compartment; EGFP: Enhanced green fluorescent protein; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; FRAP: Fluorescence recovery after photobleaching.