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. 2015 Nov 23;211(4):913–931. doi: 10.1083/jcb.201502074

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© 2015 Ittig et al.

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Figure 1. — Characterization of T3S-based protein delivery. (a) Schematic representation of T3S-dependent protein secretion into the supernatant (in vitro secretion) or eukaryotic cells (protein translocation). (b) Bacterial lysate or in vitro secretion (supernatant) of indicated strains revealed by Western blot using an anti-YopE antibody. Asterisk indicates a nonspecific band. (c) Anti-Myc immunofluorescence staining of HeLa cells infected with the indicated strains at an MOI of 100. Anti-Myc staining is shown in green and nuclei in blue. (d) Anti-Myc staining of HeLa cells infected for 45 min with the indicated strain at different MOIs. Anti-Myc staining is shown in green. Bars, 50 µm.