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. 2015 Nov 23;211(4):913–931. doi: 10.1083/jcb.201502074

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© 2015 Ittig et al.

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Figure 3. — Use of T3S-based protein delivery for functional interaction studies. (a) When coinjected, SptP abolishes the activity of SopE. HeLa cells were infected with indicated combinations of strains at indicated MOI for 1 h. After fixation, cells were stained for nuclei (blue) and F-actin (red). Translocation of YopE_1–138-SopE-Myc was monitored by an anti-Myc staining (gray images), which also stains bacteria (bright dots). Bar, 50 µm. (b) Quantification of the F-actin meshwork area per cell of images shown in panel a. (c) Quantification of the Myc staining intensity of images shown in panel a (gray images). Image analysis was performed on n = 60 cells per condition. Error bars indicate standard errors of the mean, and statistical analysis was performed using a Mann-Whitney test (***, P < 0.001; ****, P < 0.0001; ns, not significant).