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. 2015 Nov 23;211(4):913–931. doi: 10.1083/jcb.201502074

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© 2015 Ittig et al.

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Figure 5. — Delivery of EGFP and mCherry fusion proteins and nuclear relocalization. (a) T3S-based delivery of EGFP fusion proteins into cells. EGFP localization was monitored by confocal microscopy of HeLa cells infected with the indicated strains at an MOI of 100 for 4 h. Nuclei were stained with Hoechst. The YopE_1–138 fragment does not prevent the nuclear localization of translocated EGFP-NLS. Bar, 50 µm. (b) T3S-based delivery of mCherry fusion proteins into cells. mCherry localization was monitored by confocal microscopy of HeLa cells infected with the indicated strains at an MOI of 50 for 4 h. Nuclei were stained with Hoechst. The YopE_1–138 fragment does not prevent the nuclear localization of translocated NLS-mCherry. Bar, 50 µm. Fluorescent images correspond to maximum intensity z projections.