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. 2015 Nov 23;211(4):913–931. doi: 10.1083/jcb.201502074

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© 2015 Ittig et al.

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Figure 6. — Nanobody-dependent targeting of fusion proteins. (a) Nanobody-dependent sub-cellular localization of a translocated Myc-tagged fusion protein. Control HeLa and stable HeLa cell lines expressing EGFP, H2B-GFP, or EGFP-Rab2a were infected at MOI 50 with YopE_1–138-VHHGFP4-Myc-encoding bacteria for 4 h (2 h p.i., gentamicin was added) and analyzed by confocal microscopy for EGFP, Myc (stained by Alexa Fluor 647), and Hoechst for the nuclei. Arrows mark HeLa cells lacking H2B-GFP expression. Bar, 50 µm. Fluorescent images correspond to maximum intensity z projections. (b) Stable HeLa cell line expressing EGFP-Rab2a were infected as in panel a and observed at 60× by confocal microscopy for EGFP, Myc (stained by Alexa Fluor 647), and Hoechst for the nuclei. Bar, 50 µm.