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. 2015 Nov 23;211(4):815–827. doi: 10.1083/jcb.201503071

Figure 1.

Figure 1.

Kdm4b is expressed during otic placode specification/invagination. (A and B) ISH of chick embryos at stages 11 (A) and 13 (B) with an antisense probe for Kdm4b transcripts. A’ and B’ are magnifications in the region of interest. A’’ and B’’ are transverse sections, as indicated by the dotted lines in A and B. (C and D) Immunostaining with the PAX2 antibody on transverse sections through Kdm4b in situ hybridized embryos reveal overlap of PAX2 and Kdm4b expression throughout the otic ectoderm at stage 11 (C) that diminishes by stage 13 (D), when kdm4b becomes restricted to the edges. Bars, 200 µm.