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. 2015 Nov 23;211(4):775–784. doi: 10.1083/jcb.201504109

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© 2015 Ninagawa et al.

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Figure 3. — Effect of EDEM-TKO on hATF6α(C)-myct degradation. (A) Schematic structures of hATF6α, hATF6α(C)-TAP, hATF6α(C)-myct, and its three deletion mutants. bZIP and TMD denote basic leucine zipper and transmembrane domains, respectively. N indicates a potential glycosylation site. Both the amino acids 111–119 and 182–194 are expected to form an α-helix. (B) Immunoblotting of cell lysates prepared from WT and gEDEM-TKO cells expressing one of the four hATF6α(C) derivatives by transfection with or without EndoH treatment using anti–c-myc. (C–F) Cycloheximide chase of WT and gEDEM-TKO cells expressing one of the four hATF6α(C) derivatives by transfection using anti–c-myc (n = 3). Means ± SD are shown. *, P < 0.05; **, P < 0.01.