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. 2015 Nov 23;211(4):785–793. doi: 10.1083/jcb.201506103

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© 2015 Leung et al.

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Figure 2. — SUMO E3 ligases Siz1 and Siz2 and the SUMO-protease Ulp2 play opposing roles in regulating Zip1 assembly and SUMOylation of Ecm11. (A) SUMOylation of Ecm11 at the 22-h time point was analyzed as in Fig. 1. SPM, sporulation media. (B) Meiotic chromosomes were spread and visualized for indicated proteins and DNA (DAPI). Cells at the 20-h time point are shown. Bar, 5 µm. (C) Zip1 localization pattern was quantitatively analyzed for cells 20 h into meiosis. See Fig. S2 C and Materials and methods for details. Red bars, median values. *, P < 0.001 (Mann-Whitney test), in comparison to wild type. (D) SUMOylation of Ecm11 was analyzed as in A. Dots indicate the location of Ecm11 attached with polySUMO chains longer than those in wild type. (E) Meiotic chromosomes were analyzed as in B. White arrowhead, PC. Bar, 5 µm. (F) PC size was quantitatively analyzed (Materials and methods). In wild type and ulp1-mn, PC formation was barely seen. Red bars, median values. NA, not applicable. *, P < 0.001 (Mann-Whitney test) in comparison to the spo11 mutant.