Fig. 3.
Expression of Myc-LCO related genes encoding signaling components in the M. truncatula wild type and in four symbiotic mutants. Subset of 28 genes encoding transcription factors or signaling-related proteins and being rapidly activated in M. truncatula wild-type roots after a 6 h treatment with 10−8 M sMyc-LCOs (sMyc), 10−7 M nsMyc-LCOs (nsMyc), or a mixture of both (s/nsMyc). None of these genes is significantly activated by 10−8 M Nod-LCOs (Nod) after 6 h [7]. Expression responses after 6 h in the wild type (WT) as well as the nfp-1 (NFP), dmi3 (DMI3), nsp1-1 (NSP1), and ram1-1 (RAM1) mutants is visualized as a heat map, with shades of red representing up- and shades of green down-regulation. An at least 1.5-fold (p < 0.05) up-regulation is indicated by a white dot. Expression data were scaled to the maximum of a 2.5-fold regulation using the Genesis software [61]