Table 1.
Detailed information on the selection of primers for real-time reverse-transcriptase polymerase chain reaction
| Gene | NCBI ID # | Forward | Reverse | ||
|---|---|---|---|---|---|
| Primer | Sequence | Primer | Sequence | ||
| IL1β | NM_008361.3 | mIL1β-F1 | CTATACCTGTCCTGTCTA | mIL1β-R1 | GCTCTTGACTTCTATCTTG |
| IL6 | NM_031168.1 | mIL6-F1 | CTGAAACTTCCAGAGATA | mIL6-R1 | TTCATGTACTCCAGGTAG |
| TNFα | NM_013693.2 | mTNFα-F1 | TTCTGTCTACTGAACTTC | mTNFα-R1 | CCATAGAACTGATGAGAG |
| IL10 | NM_010548.2 | mIL10-F1 | CTATGCTGCCTGCTCTTA | mIL10-R1 | GCTGGTCCTTTGTTTGAAA |
| TGFβ | NM_009367.3 | mTGFβ-F1 | AGAGAAGAACTGCTGTGT | mTGFβ-R1 | GGTTGTGTTGGTTGTAGAG |
| GAPDH | NM_008084.2 | mGAPDH-F1 | GTGAAGGTCGGTGTGAAC | mGAPDH-R1 | AATCTCCACTTTGCCACTG |
NCBI National Center for Biotechnology Information
IL interleukin
TNF tumor necrosis factor
TGF transforming growth factor
GAPDH glyceraldehyde 3-phosphate dehydrogenase