Table 1.

Primers used for PCR amplification of bpsl1375 and site directed mutagenesis of bpsl1375

Primer name	Primer Sequence (5’-3’)
BPSL1375_F	CACCATGCGAGAACTGCCGA
BPSL1375_R	GCGCGGCAGCGGATCGCTCA
BPSL1375_R54K_F	GAAGCGCAGCGGCTGAAGCACAGTGTGTTCGCC
BPSL1375_R54K_R	GGCGAACACACTGTGCTTCAGCCGCTGCGCTTC
BPSL1375_D75E_F	GGCCTCGACGTCGAGCCGTTCGACC
BPSL1375_D75E_R	GGTCGAACGGCTCGACGTCGAGGCC
BPSL1375_D78E_F	GACGTCGATCCGTTCGAGCCGTACTGC
BPSL1375_D78E_R	GCAGTACGGCTCGAACGGATCGACGTC
BPSL1375_R99K_F	TGAAGGTCGTCGGCACCTATAAGGTGCTGCCGCC
BPSL1375_R99K_R	GGCGGCAGCACCTTATAGGTGCCGACGACCTTCA
BPSL1375_E132D_F	CGAAGATGGTCGATGTCGGCCGCTCGT
BPSL1375_E132D_R	ACGAGCGGCCGACATCGACCATCTTCG
BPSL1375_R135K_F	GATGGTCGAAGTCGGCAAGTCGTGCGTGCATCGCG
BPSL1375_R135K_R	CGCGATGCACGCACGACTTGCCGACTTCGACCATC