Figure 2.

Effects of single (A) or combined treatment (B) with dsRNA of opUV , opB , and opLW on mRNA levels of opsin genes in the adult cricket compound eye at ZT 6. DsRed2 was used as a negative control. The mRNA levels were measured by quantitative real-time RT-PCR. The abundance of rpl18a mRNA was used as an internal reference. The values are average of 8 compound eyes and expressed relative to those of intact crickets. The black vertical lines indicate S.E.M. One-way ANOVA was performed for each opsin genes with intact crickets, and those treated with DsRed2 RNAi and single and double RNAi of opsin genes. Values labelled by different letters significantly differ each other (P < 0.05, ANOVA followed by Tukey-test). Purple, blue, and green letters are for opUV, opB, and opLW, respectively. The dsRNAs significantly suppressed respective opsin genes. opLW mRNAs were also suppressed in opUV and opB double dsRNA treatment (B). For further explanations see text.