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. 2015 Feb 26;1:11. doi: 10.1186/s40851-015-0011-6

Table 1.

Primers for cloning and RNAi of opsin genes, and for real-time PCR of opsin and rpl18a genes

Gene Forward (5′> > 3′) Reverse (5′> > 3′)
For cloning
Opsin-UV GAGTTAATCCACATCCCGGA AGCTACAGCTTTGCAAGTGC
Opsin-Blue TGCTCGACCTGCTAATGATG ATTGCCACAGTAGCGTAGGG
Opsin-LW CCGCTCTGGCACGGCCTCCTG GTAGACGGCGTTGGCCTTGGCA
For dsRNA synthesis
Opsin-UV GCATGCATTGCCTATGACAG TGCCAAATGCACCAATTAGA
Opsin-Blue TGGTATTGGTTCTGCCATCA ATTGCCACAGTAGCGTAGGG
Opsin-LW GCGTGCTGGGAGTGATCT GCCACGTCTTGGTCAGGTAG
For real time PCR
Opsin-UV ACATTCCCCGAGCCAGA ACCAGTCCATTTCCCACGA
Opsin-Blue CTTTGCTCGACCTGCTAATGA CACAGCCTACTTCCCAACCAA
Opsin-LW CGCTCCTACATCCTCGTCTACTC CGTTCATCTTCTTGGCTTGCT
rpl18a GCTCCGGATTACATCGTTGC GCCAAATGCCGAAGTTCTTG