Fig. 1.

Workflow and expected results for the Multiplex PCR-RFLP. a: Sizes of PCR products generated in the multiplex PCR with products of 333 bp, 164 bp, and 236 bp. b: Size of restriction products generated by MaeIII restriction of the multiplex PCR products. In non-mutated samples, only the internal control of restriction in the 3460 product is restricted resulting in the removal of 26 bp from the 333 bp PCR product. c: Schematic representation of the band pattern expected from the diagnostic test. DNA samples with the 3460A, 11778A and 14484C mutations restricted with MaeIII yield the restriction products indicated. The red arrow indicates the control of restriction and the yellow arrows indicate mutation detection